to set up the software for your use. This guide shows a workflow that uses application settings. BD FACSDiva Software Quick Reference Guide for the BD LSR II. Compensation Controls in the BD FACSDiva Software Reference Manual. • Default templates are now provided for certain instrument functions. If you are the first user of the day: Boot up the computer and log on to Windows. Wait 10 seconds, then Open the BD FACSDiva software and log on. Allow the.
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Assigning Default Templates You can assign any saved template to a button on the Browser toolbar.
Getting Started with BD FACSDiva Software
If the file you are importing contains a duplicate of any existing user names, the following message appears: Refer to the Troubleshooting section in manuxl Software or Cytometer Help books.
Figure Selecting multiple objects selection handles on selected histogram main selected object selection handles on selected dot plot cursor showing object can be moved Individual selected objects can also be resized by dragging a selection handle.
About This Guide 9.
Turn on the instrument using the Green power button. Define the set of values by clicking Add Value, selecting the value in the Value field, and entering the required value.
This document contains proprietary information that More information. Automatic nd snap-to gating tools are available to create polygon and interval gates.
If more than one cell is selected, the change is made in all selected cells at the same time. For support from within Canada, call San Jose, CA If the correct version of a helper application is already installed, the installer skips to the next installation step. A selected object is highlighted in yellow Figure You can use Access to manage anything from a home inventory to a giant.
Getting Started with BD FACSDiva Software – PDF
See Inspector on page Once the gate has been drawn, its shape and size remain constant, even as new data is provided to the gate. Using the Experiment Inspector The Inspector displays experiment options when you select an experiment in the Browser. The pointer changes to orange, indicating recording is in progress. Although the experiment s contents are shown in the Browser, the plots are still blank.
Java is a trademark of Sun Microsystem, Inc. The gates are now gray because they were created in a different scaling home coordinate. Compensation cannot be calculated if PMT voltage settings are changed while recording compensation tubes. Make sure the operating system is Windows 7 bit. If the message is Cytometer Disconnected, see Electronics Troubleshooting on page for assistance.
The hierarchy in the Browser has to be followed when moving elements: When no tube-specific instrument settings exist, specimen settings apply; when no specimen-specific settings exist, experiment settings apply. InfoView User s Guide. This material briefly explains how information is stored in Windows More information.
If additional assistance is required, contact your local BD Biosciences technical support representative or supplier. Note that when you assign an analysis template as the default global worksheet, the assigned template is added to each new blank experiment. Instrument settings can apply to tubes, specimens, or experiments. Note that specimens can be exported as templates only from open experiments.
Zoom-In button—magnifies an area of a plot.
Label-specific Not labeled Label must be chosen in dialog box that appears. Tip Multiple worksheet objects can be selected for export. The menu lists all global worksheets in your experiment. To export and import user profiles, you must have administrative access.
Use the status bar at the bottom of the workspace to review cytometer connection status, fluidics information, and more. Note that templates can be exported only from open experiments. For more search options, see Maanual Saved Data on page Getting Connected More information.
For more information, see Gates on page If no tube exists, clicking Next Tube creates a new tube by duplicating the previous tube without data. Use controls in each tab to edit or adjust instrument settings.
All configuration management tasks such as set current, create, edit, and delete are done through the new Cytometer Setup and Tracking feature.